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. 2008 Jan;5(1):41-3.
doi: 10.1038/nmeth1143. Epub 2007 Dec 9.

Bottom-up genome assembly using the Bacillus subtilis genome vector

Mitsuhiro Itaya  1 , Kyoko Fujita, Azusa Kuroki, Kenji Tsuge
Affiliations

Bottom-up genome assembly using the Bacillus subtilis genome vector

Mitsuhiro Itaya et al. Nat Methods. 2008 Jan.

Abstract

We established a protocol to construct complete recombinant genomes from their small contiguous DNA pieces and obtained the genomes of mouse mitochondrion and rice chloroplast using a B. subtilis genome (BGM) vector. This method allows the design of any recombinant genomes, valuable not only for fundamental research in systems biology and synthetic biology but also for various applications in the life sciences.

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  • Life from information.
    Voigt CA. Voigt CA. Nat Methods. 2008 Jan;5(1):27-8. doi: 10.1038/nmeth0108-27. Nat Methods. 2008. PMID: 18165804 No abstract available.

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