Use of fetuin before and during vitrification of bovine oocytes
- PMID: 18069945
- DOI: 10.1111/j.1439-0531.2007.00905.x
Use of fetuin before and during vitrification of bovine oocytes
Abstract
After vitrification of oocytes, fertilization rates and subsequent development are unsatisfactory, possibly due in part to zona hardening. Foetal calf serum (FCS) can prevent zona hardening because of its fetuin content, but FCS composition varies among batches, and may contain viruses. In this study, we therefore compared media supplemented with different sources of macromolecules, 2% bovine serum albumin (BSA), 2% BSA + 1 mg/ml fetuin and 20% FCS, for handling oocytes for 10-30 min prior to vitrification. None of the treatments resulted in developmental rates comparable with the non-vitrified controls, but FCS inclusion in pre-vitrification handling medium resulted in higher blastocyst production per oocyte (p < 0.05) (10.8%) on day 9 of culture than BSA (5.3%) or BSA + fetuin (6.4%). Blastocysts developing from oocytes from all vitrification treatments were somewhat retarded relative to those developed from non-vitrified oocytes. We also tested the use of fetuin during vitrification as well as two different exposure times with cryoprotectants, 180 and 30 s. There was no significant effect of fetuin or exposure time on rates of subsequent blastocyst production.
Similar articles
-
Effects of fetuin on zona pellucida hardening, fertilization and embryo development in cattle.Anim Reprod Sci. 2002 Jun 17;71(3-4):181-91. doi: 10.1016/s0378-4320(02)00045-3. Anim Reprod Sci. 2002. PMID: 12047927
-
Effect of macromolecules in solutions for vitrification of mature bovine oocytes.Theriogenology. 2007 Mar 15;67(5):919-30. doi: 10.1016/j.theriogenology.2006.09.044. Epub 2006 Dec 18. Theriogenology. 2007. PMID: 17175017
-
Calcium concentration in vitrification medium affects the developmental competence of in vitro matured ovine oocytes.Theriogenology. 2011 Mar 1;75(4):715-21. doi: 10.1016/j.theriogenology.2010.10.012. Epub 2010 Dec 7. Theriogenology. 2011. PMID: 21144566
-
Methodological approaches for vitrification of bovine oocytes.Zygote. 2021 Feb;29(1):1-11. doi: 10.1017/S0967199420000465. Epub 2020 Sep 4. Zygote. 2021. PMID: 32885775 Review.
-
Cryopreservation of bovine oocytes: current status and recent developments.Reprod Nutr Dev. 2003 Jul-Aug;43(4):325-30. doi: 10.1051/rnd:2003024. Reprod Nutr Dev. 2003. PMID: 14971824 Review.
Cited by
-
High survival of mouse oocytes using an optimized vitrification protocol.Sci Rep. 2016 Jan 19;6:19465. doi: 10.1038/srep19465. Sci Rep. 2016. PMID: 26781721 Free PMC article.
-
Oocyte cryopreservation: searching for novel improvement strategies.J Assist Reprod Genet. 2013 Jul;30(7):865-75. doi: 10.1007/s10815-013-0028-8. Epub 2013 Jun 19. J Assist Reprod Genet. 2013. PMID: 23779099 Free PMC article. Review.
-
Follicular extracellular vesicles enhance meiotic resumption of domestic cat vitrified oocytes.Sci Rep. 2020 May 25;10(1):8619. doi: 10.1038/s41598-020-65497-w. Sci Rep. 2020. PMID: 32451384 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources