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. 2008 Feb;52(2):606-11.
doi: 10.1128/AAC.01216-07. Epub 2007 Dec 10.

Complete DNA sequence and analysis of the transferable multiple-drug resistance plasmids (R Plasmids) from Photobacterium damselae subsp. piscicida isolates collected in Japan and the United States

Mi-Jung Kim  1 , Ikuo Hirono, Ken Kurokawa, Takeshi Maki, John Hawke, Hidehiro Kondo, Mudjekeewis D Santos, Takashi Aoki
Affiliations

Complete DNA sequence and analysis of the transferable multiple-drug resistance plasmids (R Plasmids) from Photobacterium damselae subsp. piscicida isolates collected in Japan and the United States

Mi-Jung Kim et al. Antimicrob Agents Chemother. 2008 Feb.

Abstract

Photobacterium damselae subsp. piscicida is a bacterial fish pathogen that causes a disease known as pasteurellosis. Two transferable multiple-drug resistance (R) plasmids, pP99-018 (carrying resistance to kanamycin, chloramphenicol, tetracycline, and sulfonamide) and pP91278 (carrying resistance to tetracycline, trimethoprim, and sulfonamide), isolated from P. damselae subsp. piscicida strains from Japan (P99-018) and the United States (P91278), respectively, were completely sequenced and analyzed, along with the multiple-drug resistance regions of three other R plasmids also from P. damselae subsp. piscicida strains from Japan. The sequence structures of pP99-018 (150,057 bp) and pP91278 (131,520 bp) were highly conserved, with differences due to variation in the drug resistance and conjugative transfer regions. These plasmids, shown to be closely related to the IncJ element R391 (a conjugative, self-transmitting, integrating element, or constin), were divided into the conjugative transfer, replication, partition, and multiple-drug resistance regions. Each of the five multiple-drug resistance regions sequenced exhibited unique drug resistance marker composition and arrangement.

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Figures

FIG. 1.
FIG. 1.
Comparison of DNA sequences of R plasmids pP99-018 and pP91278. Regions of plasmids are indicated as drug resistance determinants (multiple-drug resistance regions); P (partition region); TRAs (transfer regions); and tnp (transposase gene), found only in pP99-018. Conserved regions are shown by gray shading.
FIG. 2.
FIG. 2.
Relationship between the putative transfer genes of pP99-018, pP91278, and homologs present in related plasmid and conjugative mobile elements. The names of the transfer genes and transfer regions are indicated. Genes are transcribed in the direction indicated by the respective arrows. Dotted lines show homologous genes. Not drawn to scale.
FIG. 3.
FIG. 3.
Comparison of the multiple-drug resistance regions of R plasmids from Japan (pSP9351, pSP98048, pSP98026, pP9014, and pP99-018) and U.S. (pP91278) isolates. Broken lines represent unknown sequences.
FIG. 4.
FIG. 4.
Electrophoretic profiles of four transferable R plasmids from P. damselae subsp. piscicida strains from Ehime and Kyushu, Japan, following PstI restriction. The white arrow points to the location of the multiple-drug resistance region in pP99-018.
See this image and copyright information in PMC

References

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