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. 1999 Jul 15;24(14):969-71.
doi: 10.1364/ol.24.000969.

Combined scanning optical coherence and two-photon-excited fluorescence microscopy

E Beaurepaire, L Moreaux, F Amblard, J Mertz

Combined scanning optical coherence and two-photon-excited fluorescence microscopy

E Beaurepaire et al. Opt Lett. .

Abstract

We demonstrate simultaneous imaging by optical coherence microscopy (OCM) and two-photon-excited (TPE) fluorescence microscopy. A mode-locked Ti:sapphire laser is focused and scanned in three dimensions through a fixed sample, generating both backscattered light and fluorescence light, which are independently detected. Both imaging modes provide rapid en-face imaging with submicrometer resolution. High-power delivery into the sample yields an OCM sensitivity in excess of 130 dB at 100-kHz pixel rates. Simultaneous imaging of cell nuclei with OCM and TPE is demonstrated in live drosophila embryos.

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