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. 2007 Dec 1;63(Pt 12):1067-9.
doi: 10.1107/S1744309107058563. Epub 2007 Nov 30.

Preliminary X-ray crystallographic studies of a tetrameric phospholipase A2 formed by two isoforms of crotoxin B from Crotalus durissus terrificus venom

D P Marchi-Salvador  1 , L C Corrêa, G H M Salvador, A J Magro, C Z Oliveira, J Iulek, A M Soares, M R M Fontes
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Preliminary X-ray crystallographic studies of a tetrameric phospholipase A2 formed by two isoforms of crotoxin B from Crotalus durissus terrificus venom

D P Marchi-Salvador et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. .

Abstract

Crotoxin B is a basic phospholipase A2 found in the venom of Crotalus durissus terrificus and is one of the subunits that constitute crotoxin. This heterodimeric toxin, which is the main component of C. d. terrificus venom, is completed by an acidic, nontoxic and non-enzymatic component (crotoxin A) and is involved in important envenomation effects, such as neurological disorders, myotoxicity and renal failure. Although crotoxin was first crystallized in 1938, no crystal structure is currently available for crotoxin, crotoxin A or crotoxin B. In this work, the crystallization, X-ray diffraction data collection to 2.28 A resolution and molecular-replacement solution of a novel tetrameric complex formed by two dimers of crotoxin B isoforms (CB1 and CB2) is presented.

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Figures

Figure 1
Figure 1
Crystal of crotoxin B from C. d. terrificus.
Figure 2
Figure 2
Tetrameric structure of crotoxin B from C. durissus terrificus shown as a ribbon diagram. The monomers of isoforms CB1 and CB2 are shown in red and blue, respectively. This figure was generated using PyMOL (DeLano, 2002 ▶).
See this image and copyright information in PMC

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