Three-dimensional microscopy with phase-shifting digital holography
- PMID: 18087480
- DOI: 10.1364/ol.23.001221
Three-dimensional microscopy with phase-shifting digital holography
Abstract
We applied phase-shifting digital holography to microscopy by deriving the complex amplitude of light scattered from microscopic three-dimensional objects through a microscope objective by video camera recording, phase-shifting analysis, and computer reconstruction. This method requires no mechanical movement and provides a flexible display and quantitative evaluation of the reconstructed images. A theory of image formation and experimental verification with specimens are described.
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