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. 1998 Aug 1;23(15):1221-3.
doi: 10.1364/ol.23.001221.

Three-dimensional microscopy with phase-shifting digital holography

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Three-dimensional microscopy with phase-shifting digital holography

T Zhang et al. Opt Lett. .

Abstract

We applied phase-shifting digital holography to microscopy by deriving the complex amplitude of light scattered from microscopic three-dimensional objects through a microscope objective by video camera recording, phase-shifting analysis, and computer reconstruction. This method requires no mechanical movement and provides a flexible display and quantitative evaluation of the reconstructed images. A theory of image formation and experimental verification with specimens are described.

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