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. 2007 Dec;35(7):952-7.
doi: 10.1080/01926230701748198.

The demonstration of immunohistochemical biomarkers in methyl methacrylate-embedded plucked human hair follicles

Kevin J Randall  1 , John R Foster
Affiliations

The demonstration of immunohistochemical biomarkers in methyl methacrylate-embedded plucked human hair follicles

Kevin J Randall et al. Toxicol Pathol. 2007 Dec.

Abstract

Plucked human hair follicles have been proposed as a potential surrogate for tumour tissue for measuring the effect of drugs on pharmacodynamic biomarkers in drug intervention studies. We describe a new technique of embedding plucked hair follicles in the acrylic resin, methyl methacrylate, and the immunohistochemical demonstration of six potential biomarkers (Ki67, EGFR, phospho-p27, phospho-histone H3, phospho-MAPK and phospho-Rb) in de-plasticised sections. The advantages of this technique over those that have been used in support of clinical drug trials, such as skin and tumour biopsies, whole blood and whole hair samples is discussed.

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  • Findings of Research Misconduct.
    [No authors listed] [No authors listed] Fed Regist. 2013 Aug 6;78(151):47699. Fed Regist. 2013. PMID: 27737229 Free PMC article. No abstract available.

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