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. 1991 Nov 22;586(2):259-63.
doi: 10.1016/0021-9673(91)85130-8.

Chromatographic separation of mixed peptides from amino acids in biological digests with volatile buffers

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Chromatographic separation of mixed peptides from amino acids in biological digests with volatile buffers

I P Armstead et al. J Chromatogr. .

Abstract

Two chromatographic methods, capable of separating mixed peptides from contaminating amino acids in biological digests, are described. Both methods involve separation on copper-Chelex resin, but each uses a different set of elution buffers. When separation method 1 was applied to a commercially available proteolytic digest of casein, the free amino acid content was reduced from 26.0% to 0.5%. With an enzymic digest of 14C-labelled proteins derived from plant biomass, separation method 2 decreased the contaminating free amino acids from 20.3% to 1.9%. Since the separated peptides are eluted with volatile buffers, they are suitable as substrates for biological experiments.

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