[An effective method for site-directed mutagenesis in plasmids and cloning single-stranded DNA fragments]
[Article in
Russian]
- PMID: 1811543
Item in Clipboard
[An effective method for site-directed mutagenesis in plasmids and cloning single-stranded DNA fragments]
[Article in
Russian]
Bioorg Khim.
1991 Nov.
Abstract
A three primer variant of the earlier devised oligonucleotide-directed mutagenesis in plasmids is described, useful also for the fast cloning of single-stranded DNA products of the asymmetric polymerase chain reaction (PCR). Using this method for plasmid pHD-001-14-11, a 59 b. p. deletion and a 7 b. p. insertion were simultaneously introduced at 81% frequency, and the PCR-copied phage fd transcription terminator (26 b. p.) was inserted with the yield of 67%.
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