Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Apr 15;164(1):67-74.
doi: 10.1042/bj1640067.

Catecholamine-sensitive adenylate cyclase of caudate nucleus and cerebral cortex. Effects of guanine nucleotides

P V Sulakhe, N L Leung, A T Arbus, S J Sulakhe, S H Jan, N Narayanan

Catecholamine-sensitive adenylate cyclase of caudate nucleus and cerebral cortex. Effects of guanine nucleotides

P V Sulakhe et al. Biochem J. .

Abstract

1. GTP and GMP-P(NH)P (guanyl-5'-yl imidodiphosphate) were observed to increase the stimulation of neural adenylate cyclase by dopamine (3,4-dihydroxyphenethylamine) and noradrenaline. 2. GMP-P(NH)P had a biphasic effect on the enzyme activity. 3. Preincubation of membranes with GMP-P(NH)P activated the enzyme by a process dependent on time and temperature. Catecholamines increased the speed and the extent of this activation. 4. Membrane fractions contained high- and low-affinity sites for GMP-P(NH)P binding: this binding was due to protein(s) of the membrane preparations. 5. Low-affinity-site binding of GMP-P(NH)P appeared to be related to the stimulatory effect on the adenylate cyclase activity.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. Proc Natl Acad Sci U S A. 1975 May;72(5):1739-43 - PubMed
    1. Biochem Biophys Res Commun. 1976 Jun 7;70(3):885-92 - PubMed
    1. Biochem J. 1976 Sep 15;158(3):535-41 - PubMed
    1. J Biol Chem. 1975 Feb 10;250(3):867-76 - PubMed
    1. J Biol Chem. 1975 Jun 10;250(11):4239-45 - PubMed