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. 2008 Jan 1;33(1):22-4.
doi: 10.1364/ol.33.000022.

Simultaneous imaging of human cone mosaic with adaptive optics enhanced scanning laser ophthalmoscopy and high-speed transversal scanning optical coherence tomography

M Pircher  1 , R J Zawadzki, J W Evans, J S Werner, C K Hitzenberger
Affiliations

Simultaneous imaging of human cone mosaic with adaptive optics enhanced scanning laser ophthalmoscopy and high-speed transversal scanning optical coherence tomography

M Pircher et al. Opt Lett. .

Abstract

We describe a novel instrument capable of acquiring, simultaneously, adaptive optics enhanced scanning laser ophthalmoscopy and optical coherence tomography (OCT) images of the human cone mosaic in vivo. The OCT system is based on transversal scanning of the sample with a line scan rate of 14 kHz, approximately 20 times faster than a previously reported instrument. We demonstrate the capability of this instrument with the measurement of the human cone spacing in perifoveal retina.

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Figures

Fig. 1
Fig. 1
Sketch of the instrument. SLD, superluminescent diode; AOM, acousto-optic modulator; TS, motorized translation stage; DC, prism for dispersion compensation.
Fig. 2
Fig. 2
Images recorded at 0.5° nasally from the fovea. (a) SLO image (average of nine frames), (b) OCT image retrieved from the IS/OS junction, (c) OCT image retrieved from the ETPR layer.
Fig. 3
Fig. 3
Two-dimensional FFT of SLO and OCT images, respectively. (a) FFT of Fig. 2(a) (arrows point to locations with increased intensity), (b) FFT of Fig. 2(b), (c) FFT obtained from SLO image recorded at 2° eccentricity, (d) FFT obtained from OCT image (ETPR layer) recorded at 2° eccentricity.
Fig. 4
Fig. 4
Cone spacing depending on the eccentricity from the fovea. SLO cone spacing retrieved from the SLO channel, OCT-ETPR cone spacing retrieved from an imaging depth corresponding to the end tips of photoreceptors, OCT-IS/OS cone spacing retrieved from an imaging depth corresponding to the junction between the IS and OS of photoreceptors; solid curve represents values retrieved from histology [12].
See this image and copyright information in PMC

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