Review
doi: 10.1016/j.chroma.2007.10.114.
Epub 2007 Dec 4.
Non-covalent capillary coatings for protein separations in capillary electrophoresis
Affiliations
- PMID: 18164023
- DOI: 10.1016/j.chroma.2007.10.114
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Review
Non-covalent capillary coatings for protein separations in capillary electrophoresis
J Chromatogr A.
.
Abstract
Adsorption of proteins, particularly basic proteins onto fused silica capillaries severely degrades capillary electrophoretic performance. This review provides a synopsis of the fundamentals underlying protein adsorption and its impact on CE performance. The efficacy of small molecule background electrolyte additives, surfactants, physically adsorbed polymers (dynamic and static), and successive multiple ionic-polymer layer coatings are evaluated using a number of performance metrics. Peak efficiency and migration time reproducibility are used as measures of reversible protein adsorption, while protein recovery, electroosmotic flow reproducibility and step changes in the baseline are used as indicators of irreversible protein adsorption.
Comment in
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Editorial on "Non-covalent capillary coatings for protein separations in capillary electrophoresis" by C.A. Lucy, A.M. MacDonald and M.D. Gulcev.J Chromatogr A. 2008 Mar 14;1184(1-2):80. doi: 10.1016/j.chroma.2007.11.068. Epub 2007 Nov 28. J Chromatogr A. 2008. PMID: 18068713 No abstract available.
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