Comparative Study
doi: 10.1016/j.jmb.2007.08.066.
Epub 2007 Sep 5.
Structure of an archaeal RNA polymerase
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- PMID: 18164030
- DOI: 10.1016/j.jmb.2007.08.066
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Comparative Study
Structure of an archaeal RNA polymerase
J Mol Biol.
.
Abstract
Related multisubunit RNA polymerases (RNAPs) carry out gene transcription in all kingdoms of life. Since structural information is limited to bacterial and eukaryotic RNAPs, we determined the cryo-electron microscopic structure of the RNAP from the thermophilic archaeon Pyrococcus furiosus at 13 A resolution. Comparison with eukaryotic RNAP II reveals a conserved architecture, no homologues for subunits Rpb8 and Rpb9, and significant deviation in the polymerase foot, jaws, pore, and protrusion. The structural organization of the archaeal RNA polymerase serves as a reference for future structure-function analysis of the transcription mechanism and allows for evolutionary comparisons.
Comment in
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Structural evolution of multisubunit RNA polymerases.Trends Microbiol. 2008 Jun;16(6):247-50. doi: 10.1016/j.tim.2008.03.008. Epub 2008 May 28. Trends Microbiol. 2008. PMID: 18468900
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