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. 2007 Dec;45(4):295-9.
doi: 10.3347/kjp.2007.45.4.295.

Loss of infectivity of Neospora caninum oocysts maintained for a prolonged time

Rosangela Soares Uzeda  1 , Kattyanne De Souza Costa, Sara Lima Santos, Alexandre Moraes Pinheiro, Maria Angela Ornelas De Almeida, Milton M McAllister, Luis Fernando Pita Gondim
Affiliations

Loss of infectivity of Neospora caninum oocysts maintained for a prolonged time

Rosangela Soares Uzeda et al. Korean J Parasitol. 2007 Dec.

Abstract

The purpose of this study was to investigate whether sporulated Neospora caninum oocysts, which had been stored for 46 mo in a 2% sulfuric acid solution at 4 degrees C, remain morphologically viable and infective to gerbils (Meriones unguiculatus). Six gerbils were orally inoculated with doses of 400 or 1,200 oocysts. Two mo after inoculation, the animals did not show any clinical signs, had no histological lesions, and were seronegative for N. caninum at 1: 50 in an immunofluorescent antibody test. PCR using the brain from each gerbil did not reveal N. caninum specific DNA. We conclude that oocysts preserved for 46 mo are not infective, despite being morphologically intact.

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Figures

Fig. 1
Fig. 1
PCR for Neospora caninum using Np21-Np6 primers (Yamage et al., 1996). Lanes (1) A 100 bp DNA ladder; (2) Negative control; (3) Empty lane; (4-5) Brains from 2 gerbils infected with N. caninum tachyzoites; (6) Empty lane; (7) N. caninum DNA (positive control).
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