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. 2008 Mar;46(3):991-5.
doi: 10.1128/JCM.00698-07. Epub 2008 Jan 3.

Epidemiological characteristics of methicillin-resistant Staphylococcus aureus isolates from children with eczematous atopic dermatitis lesions

Hee-Jung Chung  1 , Hong-Seon Jeon, Heungsup Sung, Mi-Na Kim, Soo-Jong Hong
Affiliations

Epidemiological characteristics of methicillin-resistant Staphylococcus aureus isolates from children with eczematous atopic dermatitis lesions

Hee-Jung Chung et al. J Clin Microbiol. 2008 Mar.

Abstract

In this study, we investigated the rate of colonization of skin of children with atopic dermatitis (AD) by methicillin-resistant Staphylococcus aureus (MRSA) and characterized the isolates. Active skin lesions in pediatric AD patients were cultured with Rodac Staph (Komed, Korea). S. aureus isolates were examined for drug susceptibilities, analyzed for the eta, etb, tst, and pvl genes, and typed using agr polymorphism, pulsed-field gel electrophoresis of SmaI-restricted chromosomal DNA, and staphylococcal cassette chromosome mec (SCCmec) typing. Eighty-seven (75.4%) of 115 patients had cultivable S. aureus isolates, 16 of which (18.3%) were MRSA. All MRSA isolates were susceptible to chloramphenicol, rifampin, cotrimoxazole, and ciprofloxacin. While methicillin-susceptible S. aureus (MSSA) isolates were composed of 23 isolates of singular types and nine clusters comprising 48 isolates, MRSA isolates were typed into three clones: eight isolates of pulsotype A-agr-1-SCCmec IV, five isolates of pulsotype B-agr-3-SCCmec IIb-etb positive, and three isolates of pulsotype C-agr-3-SCCmec IV. Three SCCmec IVA MRSA isolates were tst positive, but none were positive for the pvl or eta gene. Among 71 MSSA isolates, 7 isolates were tst positive, 6 of which were pulsotype F-agr-3, and 9 of 10 agr-4 isolates were eta positive. The average ages of patients carrying MSSA, SCCmec IVA MRSA, and SCCmec IIb MRSA were 7.7 +/- 4.6, 3.1 +/- 1.5, and 8.2 +/- 3.1 years, respectively. Among the patients carrying MRSA, two patients had been treated with oral antimicrobials, and one had been admitted to the hospital 18 months previously. In conclusion, community-acquired MRSA isolates of a few clones colonized the skin of patients with AD without risk factors for the acquisition of hospital-acquired MRSA, which suggested that the skin of children with AD may represent a significant reservoir of MRSA colonization in the community.

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Figures

FIG. 1.
FIG. 1.
Cluster analysis of pulsotype, agr polymorphism, SCCmec typing, and toxin gene profiles of 87 S. aureus isolates according to agr polymorphism.
See this image and copyright information in PMC

References

    1. Akiyama, H., O. Yamasaki, J. Tada, and J. Arata. 2000. Adherence characteristics and susceptibility to antimicrobial agents of Staphylococcus aureus strains isolated from skin infections and atopic dermatitis. J. Dermatol. Sci. 23155-160. - PubMed
    1. Becker, K., R. Roth, and G. Peters. 1998. Rapid and specific detection of toxigenic Staphylococcus aureus: use of two multiplex PCR enzyme immunoassays for amplification and hybridization of staphylococcal enterotoxin genes, exfoliative toxin genes, and toxic shock syndrome toxin 1 gene. J. Clin. Microbiol. 362548-2553. - PMC - PubMed
    1. Bhattacharya, D., H. Carleton, C. J. Tsai, E. J. Baron, and F. Perdreau-Remington. 2007. Differences in clinical and molecular characteristics of skin and soft tissue methicillin-resistant Staphylococcus aureus isolates between two hospitals in Northern California. J. Clin. Microbiol. 451798-1803. - PMC - PubMed
    1. Cardona, I. D., S. H. Cho, and D. Y. Leung. 2006. Role of bacterial superantigens in atopic dermatitis: implications for future therapeutic strategies. Am. J. Clin. Dermatol. 7273-279. - PubMed
    1. Cha, H. Y., D. C. Moon, C. H. Choi, J. Y. Oh, Y. S. Jeong, Y. C. Lee, S. Y. Seol, D. T. Cho, H. H. Chang, S. W. Kim, and J. C. Lee. 2005. Prevalence of the ST239 clone of methicillin-resistant Staphylococcus aureus and differences in antimicrobial susceptibilities of ST239 and ST5 clones identified in a Korean hospital. J. Clin. Microbiol. 433610-3614. - PMC - PubMed

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