doi: 10.1038/nmeth1171.
Epub 2008 Jan 6.
Highly inclined thin illumination enables clear single-molecule imaging in cells
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- PMID: 18176568
- DOI: 10.1038/nmeth1171
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Highly inclined thin illumination enables clear single-molecule imaging in cells
Nat Methods.
2008 Feb.
Erratum in
- Nat Methods. 2008 May;5(5):455
Abstract
We describe a simple illumination method of fluorescence microscopy for molecular imaging. Illumination by a highly inclined and thin beam increases image intensity and decreases background intensity, yielding a signal/background ratio about eightfold greater than that of epi-illumination. A high ratio yielded clear single-molecule images and three-dimensional images using cultured mammalian cells, enabling one to visualize and quantify molecular dynamics, interactions and kinetics in cells for molecular systems biology.
Comment in
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How the sum of its parts gets greater than the whole.Nat Methods. 2008 Feb;5(2):133-4. doi: 10.1038/nmeth0208-133. Nat Methods. 2008. PMID: 18235433 No abstract available.
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