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. 1976 Sep 10;193(4257):1007-8.
doi: 10.1126/science.181845.

Selective in vitro growth of T lymphocytes from normal human bone marrows

D A Morgan, F W Ruscetti, R Gallo

Selective in vitro growth of T lymphocytes from normal human bone marrows

D A Morgan et al. Science. .

Abstract

Selective growth of T lymphocytes occurred when unfractionated normal human bone marrow cells were cultured with conditioned medium obtained from phytohemagglutinin-stimulated normal human lymphocytes (Ly-CM). Cultures of up to 90 percent T cells have been maintained for more than 9 months. The T cells exhibited a strict growth dependence upon Ly-CM and were consistently negative for Epstein-Barr viral information.

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