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. 2008 Jan 28:(4):480-2.
doi: 10.1039/b716115h. Epub 2007 Nov 13.

Site-specific N-terminal labelling of proteins in vitro and in vivo using N-myristoyl transferase and bioorthogonal ligation chemistry

William P Heal  1 , Sasala R Wickramasinghe, Paul W Bowyer, Anthony A Holder, Deborah F Smith, Robin J Leatherbarrow, Edward W Tate
Affiliations

Site-specific N-terminal labelling of proteins in vitro and in vivo using N-myristoyl transferase and bioorthogonal ligation chemistry

William P Heal et al. Chem Commun (Camb). .

Abstract

N-Myristoyl transferase-mediated modification with azide-bearing substrates is introduced as a highly selective and practical method for in vitro and in vivo N-terminal labelling of a recombinant protein using bioorthogonal ligation chemistry.

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