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. 2008:613:97-106.
doi: 10.1007/978-0-387-74904-4_10.

In vitro analysis of ribozyme-mediated knockdown of an ADRP associated rhodopsin mutation

Dibyendu Chakraborty  1 , Patrick Whalen, Alfred S Lewin, Muna I Naash
Affiliations

In vitro analysis of ribozyme-mediated knockdown of an ADRP associated rhodopsin mutation

Dibyendu Chakraborty et al. Adv Exp Med Biol. 2008.
No abstract available

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Figures

Fig. 1
Fig. 1
The sequence and structure of the hammerhead and hairpin ribozyme
Fig. 2
Fig. 2
Retinal structure in VPP transgenics. Histological evaluation (light microscopy) at postnatal day 20 shows retinal degeneration of the VPP+/−/Rho+/+ mice when compared to non-transgenic Rho+/+ littermate. Expression of the VPP transgene on the Rho−/− background resulted in a faster rate of photoreceptor degeneration and an absence of outer segment structures
Fig. 3
Fig. 3
Time course showing the cleavage rate of the VPP target by the ribozymes
Fig. 4
Fig. 4
Cleavage rate of the HP2 (A) and HP3 (B) hairpin ribozyme. The time courses of the cleavage reactions for the mutant and wild-type oligonucleotides are shown
Fig. 5
Fig. 5
HP2 hairpin ribozyme multiple turnover cleavage reaction. (A) An image of the autoradiogram of the HP2 multiple turnover cleavage reaction. (B) A graph of the Eadiee-Hofstee plot used to calculate the kinetic coefficients of the HP2 ribozyme
See this image and copyright information in PMC

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