Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2008:613:369-75.
doi: 10.1007/978-0-387-74904-4_43.

Alphavbeta5 integrin receptors at the apical surface of the RPE: one receptor, two functions

Emeline F Nandrot  1 , Yongen Chang, Silvia C Finnemann
Affiliations
Review

Alphavbeta5 integrin receptors at the apical surface of the RPE: one receptor, two functions

Emeline F Nandrot et al. Adv Exp Med Biol. 2008.
No abstract available

PubMed Disclaimer

Figures

Figure 1
Figure 1
Fluorescence microscopy quantification of POS phagocytosis by RPE cells in vitro and in vivo. (a) Maximal projection of confocal microscopy sections of primary wild-type mouse RPE cells in culture. RPE cells show vigorous uptake of FITC-labeled POS (white) after 1 hour of phagocytic challenge. RPE cell junctions stained with ZO-1 (gray outlines). (b–d) Cryosections of 2-month-old wild-type mice eyecups labeled with rhodopsin antibody. (b) RPE-photoreceptor outer segment interface close-up showing intact rod outer segments and opsin-positive phagosomes (bright white dots) adjacent to RPE nuclei (gray). (c–d) Low magnification view of similar stainings without nuclei illustrates that these images can be used to count phagosome numbers in the RPE. (c) One hour before light onset, the RPE cell layer shows few opsin-labeled phagosomes. (d) Two hours after light onset, the RPE cell layer shows numerous opsin-labeled phagosomes confirming the daily burst of rod POS phagocytosis by the RPE. Scale bars: 20 μm. Modified from Finnemann and Chang with permission from Humana Press Inc.
Figure 2
Figure 2
Retinal adhesion is dramatically reduced in β5 knockout mice. (a) After enucleation and lens/cornea removal, retinas are swiftly peeled off opened eyecups creating shearing forces to assess retinal adhesion. In retinas with normal retinal adhesion, apical cellular domains of RPE largely remain attached to the outer surface of the neural retina. (b) Whole-mount of peeled retina, shown outer retina up, demonstrating that β5 knockout retina retains significantly less RPE pigment than wild-type retina. (c) Quantification of RPE pigment in retina peeled at different times of day. β5 knockout retina shows reduced pigment contents and attenuated adhesiveness peak compared to wild-type retina. (d) Representative immunoblots of individual peeled retina confirming the melanin pigment results. Modified from Finnemann and Chang and from Nandrot et al. (2006) with permission from Humana Press Inc. and the American Physiological Society.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Cook B, Lewis GP, Fisher SK, Adler R. Apoptotic photoreceptor degeneration in experimental retinal detachment. Invest Ophthalmol Vis Sci. 1995;36:990–996. - PubMed
    1. Edwards RB, Szamier RB. Defective phagocytosis of isolated rod outer segments by RCS rat retinal pigment epithelium in culture. Science. 1977;197:1001–1003. - PubMed
    1. Endo EG, Yao XY, Marmor MF. Pigment adherence as a measure of retinal adhesion: dependence on temperature. Invest Ophthalmol Vis Sci. 1988;29:1390–1396. - PubMed
    1. Feeney L. Lipofuscin and melanin of human retinal pigment epithelium. Fluorescence, enzyme cytochemical, and ultrastructural studies. Invest Ophthalmol Vis Sci. 1978;17:583–600. - PubMed
    1. Finnemann SC, Bonilha VL, Marmorstein AD, Rodriguez-Boulan E. Phagocytosis of rod outer segments by retinal pigment epithelial cells requires αvβ5 integrin for binding but not for internalization. Proc Natl Acad Sci U S A. 1997;94:12932–12937. - PMC - PubMed

Publication types

LinkOut - more resources