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. 2008 Feb;5(2):155-7.
doi: 10.1038/nmeth.1176. Epub 2008 Jan 13.

High-density mapping of single-molecule trajectories with photoactivated localization microscopy

Suliana Manley  1 , Jennifer M Gillette, George H Patterson, Hari Shroff, Harald F Hess, Eric Betzig, Jennifer Lippincott-Schwartz
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Free article

High-density mapping of single-molecule trajectories with photoactivated localization microscopy

Suliana Manley et al. Nat Methods. 2008 Feb.
Free article

Abstract

We combined photoactivated localization microscopy (PALM) with live-cell single-particle tracking to create a new method termed sptPALM. We created spatially resolved maps of single-molecule motions by imaging the membrane proteins Gag and VSVG, and obtained several orders of magnitude more trajectories per cell than traditional single-particle tracking enables. By probing distinct subsets of molecules, sptPALM can provide insight into the origins of spatial and temporal heterogeneities in membranes.

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