A comparative examination of swine sera for antibody to Aujeszky virus with the conventional and a modified virus-serum neutralization test and a modified direct complement fixation test
- PMID: 181975
- PMCID: PMC8383979
- DOI: 10.1186/BF03547923
A comparative examination of swine sera for antibody to Aujeszky virus with the conventional and a modified virus-serum neutralization test and a modified direct complement fixation test
Abstract
Sera of pigs from élite breeding herds, of boars and sows collected at slaughter-houses, and of pigs from herds known to be infected, were examined for antibody to Aujeszky virus. The conventional and a modified virus-neutralizing antibody (VNA) test and a modified direct complement fixation (CF) test were employed. In simultaneous titrations of positive sera the modified VNA test gave titers approx. 4 log2 units above the titers obtained by the conventional test. The conventional VNA test was found insufficiently sensitive. Unspecific neutralization in the modified VNA test was infrequent in serum dilution 1/2 and rare in dilution 1/4. The GF tests on sera of slaughter sows and animals from known infected herds showed a remarkable consistency with the VNA tests. Inconsistent results were obtained with but few sera. Abt. 5 % of the sera could not be examined because of complement fixation with control antigen.
Til undersøgelse for Aujeszky-antistoffer indsamledes blodprøver af svin i eliteavlsbesætninger, af søer og orner på slagtehuse og af svin i besætninger, hvor Aujeszky’s sygdom havde været diagnosticeret. Den konventionelle og en modificeret virus-serum-neutralisationstest og en modificeret direkte komplemenitbindingstest blev anvendt. Ved simultane titreringer gav den modificerede neutralisationstest titre, som var fire log2-enheder højere end titrene opnået med den konventionelle test. Den konventionelle test fandtes at være utilstrækkeligt følsom. Uspecifik neutralisation med den modificerede neutralisationstest forekom ikke ofte med serumfortyndingen 1/2 og var sjælden med fortyndingen 1/4. Resultaterne fra komplementbindingstesten, som blev anvendt ved serumprøverne af slagte-søer og -orner og af svin i inficerede besætninger, viste en bemærkelsesværdig overensstemmelse med resultaterne fra undersøgelsen for virusneutraliserende antistoffer. Uoverensstemmelse sås kun ved meget få sera. Omkring fem % af prøverne kunne ikke undersøges ved komplementbindingstesten, idet de viste komplementbinding med kontrolantigenet.
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