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. 2008 Apr 1;111(7):3880-3.
doi: 10.1182/blood-2007-08-107144. Epub 2008 Jan 16.

The TNF (-308A) polymorphism is associated with microchimerism in transfused trauma patients

Ryan M Gill  1 , Tzong-Hae Lee, Garth H Utter, William F Reed, Li Wen, Dan Chafets, Michael P Busch
Affiliations

The TNF (-308A) polymorphism is associated with microchimerism in transfused trauma patients

Ryan M Gill et al. Blood. .

Abstract

Microchimerism (MC), defined as the persistence of allogeneic cells at low concentrations, is well documented in transfused trauma patients. We hypothesized that genetic polymorphisms linked to cytokine production could contribute to trauma-induced immune modulation and development of microchimerism after transfusion of trauma patients. We used high-throughput SYBR-green-based genotyping of single nucleotide polymorphisms (SNPs) to characterize 59 transfused trauma patients, with MC (n=30) and without MC (n=29), for 4 functionally significant SNPs: TNF (-308), IL 10 (-1082), IFNG (+874), and TGFB1 (+915). We then compared likelihood for development of MC and the magnitude of immune suppression among subjects with and without these selected immune response SNPs. We identified a significant association between TNF (-308A) SNP and both development of MC and diminished immune responsiveness. Hence predisposing genetic factors may explain, in part, why only a subset of trauma patients develops transfusion-associated microchimerism.

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Figures

Figure 1
Figure 1
TNF (308A) polymorphism is associated with microchimerism in transfused trauma patients (P = .02). Polymorphism frequency of (A) TNF (−308A), (B) IL 10 (−1082G), (C) TGFB1 (+915G), and (D) IFNG (+874T) SNPs. Dark bars represent transfused trauma patients who were classified as MC positive (n = 30), and light bars represent transfused trauma patients who were classified as MC negative (n = 29). Two-sided Fisher exact test was used. Panel A includes an inset table depicting the relevant raw data for TNF (308) polymorphism.
See this image and copyright information in PMC

References

    1. Reed W, Lee TH, Norris PJ, Utter GH, Busch MP. Transfusion-associated microchimerism: a new complication of blood transfusions in severely injured patients. Semin Hematol. 2007;44:24–31. - PubMed
    1. Adams KM, Nelson JL. Microchimerism: an investigative frontier in autoimmunity and transplantation. JAMA. 2004;291:1127–1131. - PubMed
    1. Nelson JL. Microchimerism: incidental byproduct of pregnancy or active participant in human health? Trends Mol Med. 2002;8:109–113. - PubMed
    1. Lee TH, Paglieroni T, Ohto H, Holland PV, Busch MP. Survival of donor leukocyte subpopulations in immunocompetent transfusion recipients: frequent long-term microchimerism in severe trauma patients. Blood. 1999;93:3127–3139. - PubMed
    1. Bianchi DW, Zickwolf GK, Weil GJ, Sylvester S, DeMaria MA. Male fetal progenitor cells persist in maternal blood for as long as 27 years postpartum. Proc Natl Acad Sci U S A. 1996;93:705–708. - PMC - PubMed

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