Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2008 Feb 7;14(5):776-81.
doi: 10.3748/wjg.14.776.

Application of an indirect immunofluorescent staining method for detection of Salmonella enteritidis in paraffin slices and antigen location in infected duck tissues

Bin Yan  1 , An-Chun Cheng, Ming-Shu Wang, Shu-Xuan Deng, Zhen-Hua Zhang, Nian-Chun Yin, Ping Cao, Sheng-Yan Cao
Affiliations

Application of an indirect immunofluorescent staining method for detection of Salmonella enteritidis in paraffin slices and antigen location in infected duck tissues

Bin Yan et al. World J Gastroenterol. .

Abstract

Aim: To detect Salmonella enteritidis (S. enteritidis) in paraffin slices and antigen location in infected duck tissues.

Methods: The rabbits were immunized with purified bacillus to obtain S. enteritidis-specific antibody, which were then extracted by the caprylic-ammonium sulphate method, purified through High-Q columns. An indirect immuno-fluorescent staining method (IFA) was established to detect the S. enteritidis antigen in paraffin slices. Detected S. enteritidis in each organ tissue of ducklings experimentally infected with S. enteritidis.

Results: The gland of Garder, heart, kidney, spleen, liver, brain, ileum, jejunum, bursa of Fabricius from S. enteritidis experimentally infected ducklings were positive or strongly positive, and the S. enteritidis antigen mainly distributed in the infected cell cytoplasm.

Conclusion: IFA is an intuitionist, sensitive and specific method in detecting S. enteritidis antigen in paraffin wax slices, and it is a good method in diagnosis and antigen location of S. enteritidis. We also conclude that the gland of Garder, heart, kidney, spleen, liver, ileum, jejunum are target organs in S. enteritidis infections of duck, and S. enteritidis is an intracellular parasitic bacterium.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Organ tissues from SE-inoculated ducks immunofluorescent stain for SE. A: Positive staining baclli are adhering to the Cardiac muscle fiber (Heart); B: Positive staining baclli are adhering to the mesenchyme between the tube of Kidney (Kidney); C: Positive staining baclli are adhering to the mesenchyme between the Hepatic cord; D: Positive staining baclli are adhering to the area of medulla of the Follicle; E: Positive staining baclli are adhering to vascular endothelial cell; F: Positive staining baclli are adhering to the mesenchyme of the tube of the gland; G, H: Positive staining bacllis are adhering to the interstitial tissue of the lamina propria in villi; I: Positive staining baclli are adhering to red medulla. (Images were acquired by using 40 x objective).
See this image and copyright information in PMC

References

    1. Chiu CH, Su LH, Chu C. Salmonella enterica serotype Choleraesuis: epidemiology, pathogenesis, clinical disease, and treatment. Clin Microbiol Rev. 2004;17:311–322. - PMC - PubMed
    1. Baskerville A, Humphrey TJ, Fitzgeorge RB, Cook RW, Chart H, Rowe B, Whitehead A. Airborne infection of laying hens with Salmonella enteritidis phage type 4. Vet Rec. 1992;130:395–398. - PubMed
    1. Fantasia M, Filetici E. Salmonella enteritidis in Italy. Int J Food Microbiol. 1994;21:7–13. - PubMed
    1. Lee WC, Sakai T, Lee MJ, Hamakawa M, Lee SM, Lee IM. An epidemiological study of food poisoning in Korea and Japan. Int J Food Microbiol. 1996;29:141–148. - PubMed
    1. Angulo FJ, Swerdlow DL. Epidemiology of human. Salmonella enterica serovar Enteritidis infections in the United States. 7th ed. Ames: Iowa State University Press. 1999:33–41.

Publication types

MeSH terms

Substances