Electrophoretic separations on microfluidic chips

Abstract

This review presents a brief outline and novel developments of electrophoretic separation in microfluidic chips. Distinct characteristics of microchip electrophoresis (MCE) are discussed first, in which sample injection plug, joule heat, channel turn, surface adsorption and modification are introduced, and some successful strategies and recognized conclusions are also included. Important achievements of microfluidic electrophoresis separation in small molecules, DNA and protein are then summarized. This review is aimed at researchers, who are interested in MCE and want to adopt MCE as a functional unit in their integrated microsystems.

Fig. 1

Pinched (top) and gated (down) electrokinetic injection for MCE. From Refs. , with permission.

Fig. 2

Tapered turn (top) and constrained turn (down) in folded microchannel. From Refs. , with permission.

Fig. 3

Immobilization of ATRP initiator and grafting of PEG on the PMMA surface. From Ref. with permission.

Fig. 4

UV graft polymerization on PDMS surface. From Ref. with permission.

Fig. 5

Chip MEKC separation of 19 amino acids. From Ref. with permission.

Fig. 6

Chip platform for CE and UV detection in B.C. Lin's Lab.

Fig. 7

Chip CGE with integrated nanovolume sample purification for DNA sequencing. From Ref. with permission.

Fig. 8

Electropherogram for Promega PowerPlex 16 allelic ladder and internal lane standard. From Ref. with permission.

Fig. 9

Chip-FLFSE of multiplexed p53 mutation with polyamide drag-tags. From Ref. with permission.

Fig. 10

Chip CGE for protein sizing. From Ref. with permission.

Fig. 11

Chip 2D (MEKC-CZE) for separations of BSA digests. From Ref. with permission.

Fig. 12

Chip concentration of protein with nanofluidic filter and photopatterned cross-linked polyacrylamide gels. From Ref. , with permission.