Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2008 Jan;21(1):3-8.
doi: 10.1080/08998280.2008.11928346.

Dendritic cells and vaccines

Ralph M Steinman  1
Affiliations

Dendritic cells and vaccines

Ralph M Steinman. Proc (Bayl Univ Med Cent). 2008 Jan.
No abstract available

PubMed Disclaimer

Figures

Figure 1
Figure 1
Ralph M. Steinman, MD, with Jacques Banchereau, PhD, director of the Baylor Institute for Immunology Research.
Figure 2
Figure 2
Three elemental R's of T cell biology: repertoire, recognition, response. Reprinted with permission from Nature Medicine (1).
Figure 3
Figure 3
Dendritic cells probing the environment in mouse lymphoid tissue. Photomicrograph courtesy of Dr. Juliana Idoyaga; reprinted with permission from Nature Medicine (1).
Figure 4
Figure 4
Hybrid anti–dendritic cell antibodies (αDEC-LACK and 33D1-LACK) efficiently trigger clonal expansion of transgenic T cells in vivo compared with the protein alone (LACK) or the control group (PBS, phosphate-buffered saline). 3 × 106 CFSE-labeled TCR transgenic cells were administered intravenously to thy-1.2+ mice 1 day before immunization intraperitoneally with graded doses of αDEC-LACK, 33D1-LACK, Ig-LACK, or LACK protein. Spleens were harvested at 3 days to enumerate thy-1.1+ T cells that had diluted CFSE. The expansion of transgenic T cells is displayed as representative CFSE dilution plots, gated on thy-1.1+ LACK transgenic CD4+ T cells in response to the indicated doses of fusion monoclonal antibody or LACK protein. Reprinted with permission from Journal of Experimental Medicine (4).
Figure 5
Figure 5
DEC-205 enhances the immune efficacy of a protein vaccine. BALB/c mice were immunized subcutaneously with graded doses of αDEC-p24 or control Ig-p24 monoclonal antibodies and maturation stimulus. Nineteen days later, we assessed the percentage of IFN-γ+ CD4+ cells in gated CD3+ splenic T cells using gag p24 peptide pools. One of three similar experiments is shown. Reprinted with permission from Journal of Experimental Medicine (5).
Figure 6
Figure 6
DEC+ (33D1−) dendritic cells polarize T cells (even in BALB/c mice) to produce IFN-γ, but IL-12 p40 is not required. IL-12 p40−/− mice and control littermates were immunized intraperitoneally as indicated on the x-axis. Three weeks later, (a) the frequency of IFN-γ+ CD4+ CD3+ splenic T cells was determined by intracellular staining or (b) CD4+ splenocytes were restimulated in vitro with CD11c+ cells, medium, or LACK peptide for 36 to 48 hours to measure IL-4–secreting cells by ELISPOT. Reprinted with permission from Journal of Experimental Medicine (4).
Figure 7
Figure 7
αCD70 treatment (FR70, H.Yagita) in vivo blocks immunity to LACK in naive mice, after targeting of antigen to DEC+ but not DEC− dendritic cells. BALB/c mice were injected intraperitoneally with 50 μg αCD70 monoclonal antibody 1 day before intraperitoneal immunization with 10 μg αDEC-LACK, 10 μg 33D1LACK, 50 μg LACK protein, and 10 μg Ig-LACK in the presence of αCD40 and poly I:C, or PBS. Three weeks later, the frequency of IFN-γ+ CD4+ CD3+ splenic T cells was determined by intracellular staining. One of two similar experiments. Reprinted with permission from Journal of Experimental Medicine (4).
See this image and copyright information in PMC

References

    1. Steinman RM. Dendritic cells: versatile controllers of the immune system. Nat Med. 2007;13(10):vii–xi. - PubMed
    1. Obeid M, Tesniere A, Ghiringhelli F, Fimia GM, Apetoh L, Perfettini JL, Castedo M, Mignot G, Panaretakis T, Casares N, Metivier D, Larochette N, van Endert P, Ciccosanti F, Piacentini M, Zitvogel L, Kroemer G. Calreticulin exposure dictates the immunogenicity of cancer cell death. Nat Med. 2007;13(1):54–61. - PubMed
    1. Dubos R. Pasteur and Modern Science. Washington, DC: American Society for Microbiology; 1995.
    1. Soares H, Waechter H, Glaichenhaus N, Mougneau E, Yagita H, Mizenina O, Dudziak D, Nussenzweig MC, Steinman RM. A subset of dendritic cells induces CD4+ T cells to produce IFN-gamma by an IL-12-independent but CD70-dependent mechanism in vivo. J Exp Med. 2007;204(5):1095–1106. - PMC - PubMed
    1. Trumpfheller C, Finke JS, Lopez CB, Moran TM, Moltedo B, Soares H, Huang Y, Schlesinger SJ, Park CG, Nussenzweig MC, Granelli-Piperno A, Steinman RM. Intensified and protective CD4+ T cell immunity in mice with anti-dendritic cell HIV gag fusion antibody vaccine. J Exp Med. 2006;203(3):607–617. - PMC - PubMed

LinkOut - more resources