doi: 10.1021/bi00661a032.
Arthrobacter luteus restriction endonuclease recognition sequence and its cleavage map of SV40 DNA
- PMID: 182213
- DOI: 10.1021/bi00661a032
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Arthrobacter luteus restriction endonuclease recognition sequence and its cleavage map of SV40 DNA
Biochemistry.
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Abstract
The nucleotide sequence at the cleavage site of the restriction endonuclease isolated from Arthrobacter luteus (Alu) has been determined. The endonuclease cleaves at the center of a palindromic tetranucleotide sequence to give even-ended duplex DNA fragments phosphorylated at the 5'-end. The endonuclease cleaves SV40 form I DNA into 32 fragments. The order and sizes of these fragments have been determined to provide an Alu cleavage map of the SV40 genome.
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