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. 2003 Nov:Chapter 4:Unit 4.13.
doi: 10.1002/0471143030.cb0413s20.

Fluorescent labeling of yeast

J J Baggett  1 , J D Shaw, C J Sciambi, H A Watson, B Wendland
Affiliations

Fluorescent labeling of yeast

J J Baggett et al. Curr Protoc Cell Biol. 2003 Nov.

Abstract

This unit describes the use of several different fluorescence methods for labeling yeast cells. It includes methods to label the vacuole, the actin cytoskeleton, and chitin deposits on cell walls (bud scars), as well as methods for visualizing specific proteins in live cells with GFP chimeras and in fixed cells by immunofluorescence.

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