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. 2008 Jan 31:1:2.
doi: 10.1186/1755-8794-1-2.

Microarray analysis in B cells among siblings with/without MS - role for transcription factor TCF2

Jagannadha R Avasarala  1 , Sridar V Chittur, Ajish D George, John A Tine
Affiliations

Microarray analysis in B cells among siblings with/without MS - role for transcription factor TCF2

Jagannadha R Avasarala et al. BMC Med Genomics. .

Abstract

Background: We investigated if global gene expression and transcription networks in B-lymphocytes of siblings with multiple sclerosis (MS) were different from healthy siblings.

Results: Using virus-transformed immortalized B cells and human whole genome bioarrays with validation using RT-qPCR, we found that in siblings with MS, genes for CXCL10, serpin B1 and FUT4 were up regulated whereas CDC5L, TNFRSF19 and HLA-DR were down regulated, among others; transcription analysis showed two intersecting clusters of transcriptional factors - the larger, governed by the upregulated transcription factor 2 (TCF2) and the smaller network regulated by the downregulated CDC5L.

Conclusion: No study has linked TCF2 to MS and to better understand the role of TCF2 in MS, studies in larger cohorts are required.

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Figures

Figure 1
Figure 1
Hierarchical clustering of all statistically significant genes that show differential expression between diseased and normal samples. Upregulated are colored red and downregulated genes are colored green. Clustering of genes is shown on the left while condition based tree is shown on the top.
Figure 2
Figure 2
Analysis of transcription networks from microarray data.
Figure 3
Figure 3
Transcription factor network deciphered from genes differentially expressed at a 1.5 fold cutoff in microarray data. The color 'red" shows upregulation while "green" shows downregulated expression. Genes are depicted by circles while transcription factors are represented as rectangles. Green arrows indicate that the nodes at each end of the arrow are regulated in the same direction (up or down) while the red arrows connect the nodes that are anticorrelated.
Figure 4
Figure 4
Results from functional profiling of data using Onto-Express. (A) The figure shows the significant (red) biological processes represented in a set of genes differentially expressed between two clinical groups. (B) The figure shows the significant (red) molecular function represented in a set of genes differentially expressed between two clinical groups.
See this image and copyright information in PMC

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