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. 2008 Feb 27;130(8):2446-7.
doi: 10.1021/ja7107867. Epub 2008 Feb 5.

Probing transient copper chaperone-Wilson disease protein interactions at the single-molecule level with nanovesicle trapping

Jaime J Benítez  1 , Aaron M Keller, Patrick Ochieng, Liliya A Yatsunyk, David L Huffman, Amy C Rosenzweig, Peng Chen
Affiliations

Probing transient copper chaperone-Wilson disease protein interactions at the single-molecule level with nanovesicle trapping

Jaime J Benítez et al. J Am Chem Soc. .
No abstract available

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Figures

Figure 1
Figure 1
Nanovesicle trapping and smFRET for studying transient protein–protein interactions.
Figure 2
Figure 2
(A and B) Fluorescence and EFRET (ICy5/(ICy3 + ICy5)) trajectories of two interacting Cy5–Hah1 and Cy3–MBD4 pairs. (C) Hah1–MBD4 interaction scheme. (D) Waiting time (τ) distributions for the six kinetic steps in (C). Data compiled from 163 Hah1–MBD4 interacting pairs and total 1101 transitions. Solid lines are exponential fits.
See this image and copyright information in PMC

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