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. 2008 Aug;57(8):1253-62.
doi: 10.1007/s00262-008-0460-2. Epub 2008 Feb 7.

The neoplastically transformed (CD30hi) Marek's disease lymphoma cell phenotype most closely resembles T-regulatory cells

L A Shack  1 , J J Buza, S C Burgess
Affiliations

The neoplastically transformed (CD30hi) Marek's disease lymphoma cell phenotype most closely resembles T-regulatory cells

L A Shack et al. Cancer Immunol Immunother. 2008 Aug.

Abstract

Introduction: Marek's disease (MD), a herpesvirus-induced lymphoma of chickens is a unique natural model of CD30-overexpressing (CD30hi) lymphoma. We have previously proposed that the CD30hi neoplastically transformed CD4+ T cells in MD lymphomas have a phenotype antagonistic to cell mediated immunity. Here were test the hypothesis that the CD30hi neoplastically transformed MD lymphoma cells have a phenotype more closely resembling T-helper (Th)-2 or regulatory T (T-reg) cells.

Materials and methods: We separated ex vivo-derived CD30hi, from the CD30lo/- (non-transformed), MD lymphoma cells and then quantified the relative amounts of mRNA and proteins for cytokines and other genes that define CD4+ Th-1, Th-2 or T-reg phenotypes.

Results and discussion: Gene Ontology-based modeling of our data shows that the CD30hi MD lymphoma cells having a phenotype more similar to T-reg. Sequences that could be bound by the MD virus putative oncoprotein Meq in each of these genes' promoters suggests that the MD herpesvirus may play a direct role in maintaining this T-reg-like phenotype.

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Figures

Fig. 1
Fig. 1
Difference in cytokine and cell antigen mRNA (a) and protein (c) expression (measured by duplex real-time reverse transcriptase PCR and proteomics as described in M and M) of CD30hi, relative to CD30lo/−, MD lymphoma cells. The amount of CD30lo/− mRNA expression is set to 100% and protein expression is set to 0. The CD30hi, were separated from the CD30lo/−, lymphoma cells by magnetic activated cell sorting. Inset: the mean purity (±SEM) was 95.7 ± 3.3 and 93.9 ± 4.9%, for the (1) CD30hi and (2) CD30lo/− cells, respectively. GO-based hypothesis-driven quantitative modeling as described in M and M for the mRNA (b) and protein (d) shows that the CD30hi cells have a T-reg phenotype
Fig. 2
Fig. 2
Difference in IL-2 and IL-18 protein distribution in CD30lo/− and CD30hi lymphoma cells mean (±SEM). Both IL-2 and IL-18 protein are preferentially distributed in the differential detergent fractions representing the least superficial areas of the cell and the less soluble proteins
See this image and copyright information in PMC

References

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