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. 2007 Oct;13(10):1565-7.
doi: 10.3201/eid1310.070570.

Isolation of Bartonella sp. from sheep blood

David A Bemis  1 , Stephen A Kania
Affiliations

Isolation of Bartonella sp. from sheep blood

David A Bemis et al. Emerg Infect Dis. 2007 Oct.

Abstract

A Bartonella sp. was isolated from sheep blood. Bacterial identification was conducted by using electron microscopy and DNA sequencing of the 16S rRNA, citrate synthase, riboflavin synthase, and RNAase P genes. To our knowledge, this is the first report of ovine Bartonella infection.

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Figures

Figure 1
Figure 1
Morphologic analysis of a Bartonella sp. isolated from sheep blood. A) Colonies growing in sheep blood surface biofilm seen in reflected light after 25 days. Scale bar = 10 mm. B) Transmission electron micrograph of a representative cell that was dispersed from a 25-day-old colony and negatively stained with 0.5% potassium phosphotungstic acid. Scale bar = 500 nm
Figure 2
Figure 2
Phylogenetic trees (DNAstar ClustalW Slow and Accurate; DNASTAR Inc., Madison, WI, USA) of 3 Bartonella genes. A) Citrate synthase. B) Riboflavin synthase. C) 16S rDNA. Sheep blood 1 is compared with species showing the highest homology for each gene.
See this image and copyright information in PMC

References

    1. Chomel BB, Boulouis HJ, Maruyama S, Breitschwerdt EB. Bartonella spp. in pets and effect on human health. Emerg Infect Dis. 2006;12:389–94. - PMC - PubMed
    1. Chang C-C, Chomel BB, Kasten RW, Heller R, Kocan KM, Ueno H, et al. Bartonella spp. isolated from wild and domestic ruminants in North America. Emerg Infect Dis. 2000;6:306–11. 10.3201/eid0603.000313 - DOI - PMC - PubMed
    1. Dehio C, Lanz C, Pohl R, Behrens P, Bermond D, Piemont Y, et al. Bartonella schoenbuchii sp. nov., isolated from the blood of wild roe deer. Int J Syst Evol Microbiol. 2001;51:1557–65. - PubMed
    1. Rolain JM, Rousset E, LaScola B, Duquesnel R, Raoult D. Bartonella schoenbuchensis isolated from the blood of a French cow. Ann N Y Acad Sci. 2003;990:236–8. 10.1111/j.1749-6632.2003.tb07370.x - DOI - PubMed
    1. Halos L, Jamal T, Maillard R, Girard B, Guillot J, Chomel B, et al. Role of Hippoboscidae flies as potential vectors of Bartonella spp. infecting wild and domestic ruminants. Appl Environ Microbiol. 2004;70:6302–5. 10.1128/AEM.70.10.6302-6305.2004 - DOI - PMC - PubMed

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