Dengue virus 3 genotype 1 associated with dengue fever and dengue hemorrhagic fever, Brazil

Abstract

Dengue serotype 3 viruses were isolated from patients in Brazil from 2002 through 2004. On the basis of phylogenetic analyses, these isolates were assigned genotype 1. This genotype had never been reported in South America before. Its appearance indicates a major risk factor for dengue epidemics and severe disease.

Figure 1

Location of the city of Belo Horizonte and its boroughs Pampulha, Nordeste, and Noroeste, Minas Gerais State, where samples of dengue virus serotype 3 were collected during 2002–2004.

Figure 2

Phylogenetic trees of established dengue virus serotype 3 (DENV-3) and new sequences from Minas Gerais State, Brazil, identified in this study. A) The tree is based on a 504-nt sequence alignment comprising the C-prM gene (nucleotides 137–638). B) The tree is based on a 1,023-nt partial E nucleotide sequences (nucleotides 1022–2008). This tree was generated by neighbor-joining using the Tamura Nei model implemented by using MEGA3 software (www.megasoftware.net). Numbers to the left of nodes represent bootstrap values (1,000 replicates) in support of grouping to the right. Numbers to the right in parentheses of branches indicate the GenBank accession number. Roman numerals denote the different genotypes of DENV-3.