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. 2008 Apr;190(8):3083-7.
doi: 10.1128/JB.01448-07. Epub 2008 Feb 8.

Plasmid transfer via transduction from Streptococcus thermophilus to Lactococcus lactis

Andreas Ammann  1 , Horst Neve, Arnold Geis, Knut J Heller
Affiliations

Plasmid transfer via transduction from Streptococcus thermophilus to Lactococcus lactis

Andreas Ammann et al. J Bacteriol. 2008 Apr.

Abstract

Using Streptococcus thermophilus phages, plasmid transduction in Lactococcus lactis was demonstrated. The transduction frequencies were 4 orders of magnitude lower in L. lactis than in S. thermophilus. These results are the first evidence that there is phage-mediated direct transfer of DNA from S. thermophilus to L. lactis. The implications of these results for phage evolution are discussed.

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Figures

FIG. 1.
FIG. 1.
(A) Agarose gel electrophoresis of intact plasmid DNA extracted from plasmid-free recipient strains S. thermophilus a10 (lane 1) and L. lactis Bu2-60 (lane 2), from S. thermophilus donor strain a10(pAG106AE) (lane 3), and from three L. lactis Bu2-60 transductants, Bu2-60-STD1, Bu2-60-STD2, and Bu2-60-STD3 (lanes 4 to 6). (B) Agarose gel electrophoresis of StyI digests of plasmid DNA extracted from S. thermophilus donor strain a10(pAG106AE) (lane 7) and from the three L. lactis Bu2-60 transductants (lanes 8 to 10). Phage lambda cut with HindIII (lane M1) and a 1-kb ladder (lane M2) were used as size references.
FIG. 2.
FIG. 2.
Transmission electron micrographs of S. thermophilus P1109 phage particles adsorbed to L. lactis Bu2-60 cells.
See this image and copyright information in PMC

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