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. 2004 Nov:Chapter 23:Unit 23.9.
doi: 10.1002/0471142727.mb2309s68.

Transgenic mouse production by zygote injection

David A Conner  1
Affiliations

Transgenic mouse production by zygote injection

David A Conner. Curr Protoc Mol Biol. 2004 Nov.

Abstract

This unit describes methods for the production of transgenic mice by injection of DNA into zygotes, including fertilized-egg isolation, zygote injection, and oviduct reimplantation. Methods for the preparation of plasmid and BAC DNA suitable for microinjection are also presented.

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