Rice JASMONATE RESISTANT 1 is involved in phytochrome and jasmonate signalling

Abstract

Jasmonic acid (JA) is an important negative regulator of light-regulated coleoptile elongation in rice. We isolated rice JASMONATE RESISTANT 1 (osjar1) mutants from the Tos17 mutant panel by BLAST search. In far-red and blue lights, osjar1 coleoptiles were longer if compared with the wild type (WT), indicating that OsJar1 participates in the suppression of coleoptile elongation in these light conditions, while the mutant did not show a clear phenotype in red light. The analysis of OsJar1 expression in phytochrome (phy) mutants revealed that phytochrome A (phyA) and phytochrome B (phyB) act redundantly to induce this gene by red light, presumably. Unexpectedly, blue light-induced expression of OsJar1 gene was impaired in phyA-deficient mutants, indicating the involvement of phyA in the blue light signalling. In WT seedlings, OsJar1 transcripts were up-regulated transiently in response to treatment with exogenous methyl-jasmonic acid (MeJA). The dose-response curve of the MeJA treatment showed a characteristic pattern: concentrations as low as 4.5 nM could induce OsJar1 transcription, while the gene was superinduced at a concentration of 450 microM MeJA. In summary, this paper demonstrated that OsJar1 modulates light and JA signalling in the photomorphogenesis of rice.