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. 2008 Mar 12;130(10):2952-3.
doi: 10.1021/ja800225k. Epub 2008 Feb 14.

Stabilization of the collagen triple helix by O-methylation of hydroxyproline residues

Frank W Kotch  1 , Ilia A Guzei, Ronald T Raines
Affiliations

Stabilization of the collagen triple helix by O-methylation of hydroxyproline residues

Frank W Kotch et al. J Am Chem Soc. .
No abstract available

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Figures

Figure 1
Figure 1
CD spectroscopy and DSC data for peptides 1 and 2. (A) CD spectra of 1 (E) and 2 (J) (100 μM) at 4 °C in 50 mM HOAc (pH 3.0). (B) Thermal denaturation of 1 and 2 (200 μM) in 50 mM HOAc(aq) (E,J) and 2:1 EG/50 mM HOAc (pH 3.0) (Å,F). (C) DSC scans of 1 (231 μM) and 2 (129 μM) in 50 mM HOAc (pH 3.0); scan rate = 15 °C/h.
Figure 2
Figure 2
(A) Molecular drawing of crystalline Ac-Mop-OMe (50% probability ellipsoids). (B) Conformation of crystalline Ac-Mop-OMe and Ac-Hyp-OMe showing the putative n→π* interaction.
See this image and copyright information in PMC

References

    1. For a review, see:Raines RT. Protein Sci. 2006;15:1219–1225.

    1. Bella J, Eaton M, Brodsky B, Berman HM. Science. 1994;266:75–81.Bella J, Brodsky B, Berman HM. Structure. 1995;3:893–906.Miles CA, Burjanadze TV. Biophys. J. 2001;80:1480–1486.

    1. It is noteworthy that the frequency of Hyp could be too low to support such a water network in natural collagen. In the strands of human type-I collagen, an Xaa–Hyp–Gly sequence occurs in no more than four consecutive triads, and occurs in four consecutive triads only twice over >1000 residues.

    1. Holmgren SK, Taylor KM, Bretscher LE, Raines RT. Nature. 1998;392:666–667.Holmgren SK, Bretscher LE, Taylor KM, Raines RT. Chem. Biol. 1999;6:63–70.DeRider ML, Wilkens SJ, Waddell MJ, Bretscher LE, Weinhold F, Raines RT, Markley JL. J. Am. Chem. Soc. 2002;124:2497–2505.

    1. Shoulders MD, Guzei IA, Raines RT. Biopolymers. 2008;89:xxx–xxx. - PMC - PubMed

Publication types