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Comparative Study
. 2008 Apr;46(4):1517-9.
doi: 10.1128/JCM.02046-07. Epub 2008 Feb 13.

Evaluation of the Abbott RealTime HBV DNA assay and comparison to the Cobas AmpliPrep/Cobas TaqMan 48 assay in monitoring patients with chronic cases of hepatitis B

Marco Ciotti  1 , Fabbio Marcuccilli, Tania Guenci, Maria Grazia Prignano, Carlo Federico Perno
Affiliations
Comparative Study

Evaluation of the Abbott RealTime HBV DNA assay and comparison to the Cobas AmpliPrep/Cobas TaqMan 48 assay in monitoring patients with chronic cases of hepatitis B

Marco Ciotti et al. J Clin Microbiol. 2008 Apr.

Abstract

The new Abbott RealTime hepatitis B virus (HBV) assay was compared to the Cobas AmpliPrep/Cobas TaqMan assay with 128 serum samples from patients with chronic hepatitis B. There was an excellent correlation (r = 0.961) between the two assays, with the Abbott RealTime test showing at least equivalent sensitivity and a slightly wider dynamic range than the Cobas TaqMan assay. By coupling high sensitivity with a large dynamic range, the Abbott RealTime HBV assay is useful in monitoring the response to antiviral therapy.

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Figures

FIG. 1.
FIG. 1.
Linear regression analysis performed on 100 paired quantitative results obtained with the two assays.
FIG. 2.
FIG. 2.
QCMD HBV06-01 panel tested with the Abbott m2000 HBV assay. The Abbott RealTime HBV results (measured) are compared to expected sample concentrations (target) and the mean results obtained by all 109 participants (consensus).
See this image and copyright information in PMC

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