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. 2008 Feb;116(2):203-8.
doi: 10.1289/ehp.10353.

HPRT mutations in lymphocytes from 1,3-butadiene-exposed workers in China

Shengxue Liu  1 , Lin Ao, Bing Du, Yanhong Zhou, Jian Yuan, Yang Bai, Ziyuan Zhou, Jia Cao
Affiliations

HPRT mutations in lymphocytes from 1,3-butadiene-exposed workers in China

Shengxue Liu et al. Environ Health Perspect. 2008 Feb.

Abstract

Background: 1,3-Butadiene (BD) is an important industrial chemical and an environmental and occupational pollutant. The carcinogenicity of BD in rodents has been proved, but its carcinogenic and mutagenic molecular mechanism(s) are not fully elucidated in humans.

Objectives: In the present study, we compared the mutation frequencies and exon deletions of BD-exposed workers with that of control subjects in China to identify the characteristic mutations associated with BD exposure in the human HPRT (hypoxanthine-guanine-phosphoribosyltransferase) gene.

Methods: Seventy-four workers exposed to BD via inhalation and 157 matched controls were evaluated in Nanjing, China. Molecular analysis of HPRT mutant T lymphocytes from BD-exposed workers and nonexposed control subjects was conducted to identify changes in the structure of the HPRT gene. A total of 783 HPRT mutants were analyzed by multiplex polymerase chain reaction, in which 368 HPRT mutants were isolated from BD-exposed workers and 415 mutants from control subjects.

Results: The BD-exposed workers showed a higher mutation frequency (18.2 +/- 9.4 x 10(-6)) than the control subjects (12.7 +/- 7.3 x 10(-6)), but the difference was not significant (p > 0.05). The frequency of exon deletions in BD-exposed workers (27.4%) was significantly higher than that in control subjects (12.5%) (p < 0.05), which mainly included multiplex exon deletions (2-8 exons).

Conclusions: The results of the present study suggest that BD should increase the frequency of large deletions of HPRT gene in human lymphocytes This change confirms and supports the previous findings in BD-exposed workers.

Keywords: 1,3-butadiene; BD; HPRT gene; exon deletion; lymphocyte; occupational exposure.

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Figures

Figure 1
Figure 1
BD exposure dose at each sampling location of the BD-exposed group.
Figure 2
Figure 2
Schematic diagram of the distribution of deleted exons in mutants from BD-exposed subjects and control subjects. (A) Control subjects. (B) BD-exposed subjects.
See this image and copyright information in PMC

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