Characterization of the thoracic duct T-helper cells that co-mediate, with antibody, the rapid expulsion of Trichinella spiralis in adult rats

Abstract

Thoracic duct cells that act synergistically with immune serum or antibody to transfer rapid expulsion of a challenge infection with Trichinella spiralis muscle larvae were characterized as OX38+, OX8-, OX22- T helper cells. Protective capacity was confined to the dividing T helpers that appeared on days 3-5 in the thoracic duct of rats during a T. spiralis infection. To realize their intestinal priming potential in recipient rats. MHC-compatibility between donor and recipient rat was required. Fractionation of immune serum with 40% saturated ammonium sulphate left transferable protective activity in both the precipitate and supernatant fractions. Absorption of immune serum with muscle larvae antigen removed the capacity to transfer protection.