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. 2008 May 15;23(10):1547-54.
doi: 10.1016/j.bios.2008.01.014. Epub 2008 Jan 18.

Amperometric immunosensor for diagnosis of BLV infection

Bogumila Kurtinaitiene  1 , Dovile Ambrozaite, Valdas Laurinavicius, Almira Ramanaviciene, Arunas Ramanavicius
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Amperometric immunosensor for diagnosis of BLV infection

Bogumila Kurtinaitiene et al. Biosens Bioelectron. .

Abstract

A new amperometric immunosensor for detection of antibodies against bovine leukemia protein (gp51) was designed. The detection of antibody-antigen complex formation was based on application of secondary antibodies labeled with horseradish peroxidase (HRP). Ferrocenecarboxylic acid (FCA) and N,N,N',N'-tetramethylbenzidine (TMB) were selected as suitable mediators for this immunosensor. Optimal conditions for amperometric detection were found. Sensitivity of created system was compared with the results of enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion (AGID) reaction, and was sufficient for detection of usual anti-gp51 antibody concentration present in the blood serum of BLV-infected cattle.

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