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. 2008 Mar;87(3):278-82.
doi: 10.1177/154405910808700308.

Diversity of Veillonella spp. from sound and carious sites in children

N Arif  1 , E C Sheehy, T Do, D Beighton
Affiliations

Diversity of Veillonella spp. from sound and carious sites in children

N Arif et al. J Dent Res. 2008 Mar.

Abstract

Detailed data on the distribution of Veillonella in caries-free and caries-active subjects are scarce. We hypothesized that the diversity of the genus would be lower in caries lesions than in plaque from caries-free individuals. The proportions of Veillonella were not significantly different in the two groups. All isolates (n = 1308) were genotyped by REP-PCR, and different genotypes (n = 170) were identified by 16S rRNA, dnaK, and rpoB sequencing. V. parvula, V. dispar, and V. atypica were in both groups, V. denticariosi only in caries lesions, and V. rogosae only from the caries-free individuals (p < 0.009). Lesions were more likely to harbor a single predominant species (p = 0.0018). The mean number of genotypes in the lesions was less than in the fissure (p < 0.001) or buccal (p = 0.011) sites. The Veillonella from caries-free sites were more diverse than those from caries lesions, and may be related to the acidic environment of caries lesions.

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Figures

Figure
Figure. Representative REP-PCR patterns obtained from 2 caries lesions in Participant 9 and from the fissure plaque from caries-free Participant 2. Lanes 1, 8, and 15 are marker lanes. Each of the other lanes represents a single isolate.
See this image and copyright information in PMC

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