Lansoprazole Novel Effector Sites Revealed by Autoradiography: Relation to Helicobacter pylori, Colon, Esophagus and Others

Abstract

Lansoprazole uptake sites by two kinds of autoradiographic procedures were compared with recent literature. The uptake sites have been seen in the Helicobacter pylori, colonic epithelial cells, inflammatory cells, peripheral autonomic nerves and enterochromaffinlike cells as well as gastric parietal cells. Each uptake sites corresponded to the reported localization of P-type ATPase or acidic compartment.

Keywords: Helicobacter pylori; autoradiography; colon; inflammatory cell; lansoprazole.

Fig. 1

Lansoprazole uptake sites in rat fundic mucosa and isolated parietal cells. Autoradiography of soluble compounds. a, b: Uptake sites are accumulated in the parietal cells. c: Electron microscopic autoradiograph showing that the silver grains showing the uptake sites are accumulated near the intracellular canaliculi of the parietal cell. d: In the isolated parietal cells, silver grains are recognized in the intracellular canaliculi. e, f: In the base of the fundic glands, silver grains are accumulated in the enterochromaffin-like cells.

Fig. 2

Lansoprazole uptake sites in H. pylori-infected gastric mucosa. a, b: In H. pylori-infected Mongolian gerbil fundic mucosa (a), the uptake sites of lansoprazole are seen in the inflammatory cells in the lamina propria mucosa as well as parietal cells and H. pylori, while in the control gerbils the uptake sites are accumulated in the luminal side of the parietal cells. Autoradiography of soluble compounds. c: By in vitro autoradiography using unfixed cryostat sections of the Mongolian gerbil fundic mucosa, the uptake sites are seen near the surface epithelial cells and inflammatory cells (arrows). d, e: By Electron microscopic autoradiography of soluble compounds, the uptake sites are accumulated in the H. pylori (d) as well as inflammatory cells (e).

Fig. 3

Lansoprazole uptake sites in control colonic mucosa. a: By in vitro autoradiography of the control rat colonic mucosa, the uptake sites of the lansoprazole are seen in the inflammatory cells (arrows) as well as in the epithelial cells. b: By autoradiography of soluble compounds, the uptake sites are mostly seen in the luminal surface of the colonic epithelial cells (arrows).