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. 2008 Feb 28;14(8):1222-30.
doi: 10.3748/wjg.14.1222.

Ameliorative effects of bombesin and neurotensin on trinitrobenzene sulphonic acid-induced colitis, oxidative damage and apoptosis in rats

Alper Akcan  1 , Sebahattin Muhtaroglu, Hulya Akgun, Hizir Akyildiz, Can Kucuk, Erdogan Sozuer, Alper Yurci, Namik Yilmaz
Affiliations

Ameliorative effects of bombesin and neurotensin on trinitrobenzene sulphonic acid-induced colitis, oxidative damage and apoptosis in rats

Alper Akcan et al. World J Gastroenterol. .

Abstract

Aim: To investigate the effects of bombesin (BBS) and neurotensin (NTS) on apoptosis and colitis in an ulcerative colitis model.

Methods: In this study, a total of 50 rats were divided equally into 5 groups. In the control group, no colitis induction or drug administration was performed. Colitis was induced in all other groups. Following the induction of colitis, BBS, NTS or both were applied to three groups of rats. The remaining group (colitis group) received no treatment. On the 11th d after induction of colitis and drug treatment, blood samples were collected for TNF-alpha and IL-6 level studies. Malondialdehyde (MDA), carbonyl, myeloperoxidase (MPO) and caspase-3 activities, as well as histopathological findings, evaluated in colonic tissues.

Results: According to the macroscopic and microscopic findings, the study groups treated with BBS, NTS and BBS + NTS showed significantly lower damage and inflammation compared with the colitis group (macroscopic score, 2.1 +/- 0.87, 3.7 +/- 0.94 and 2.1 +/- 0.87 vs 7.3 +/- 0.94; microscopic score, 2.0 +/- 0.66, 3.3 +/- 0.82 and 1.8 +/- 0.63 vs 5.2 +/- 0.78, P < 0.01). TNF-alpha and IL-6 levels were increased significantly in all groups compared with the control group. These increases were significantly smaller in the BBS, NTS and BBS + NTS groups compared with the colitis group (TNF-alpha levels, 169.69 +/- 53.56, 245.86 +/- 64.85 and 175.54 +/- 42.19 vs 556.44 +/- 49.82; IL-6 levels, 443.30 +/- 53.99, 612.80 +/- 70.39 and 396.80 +/- 78.43 vs 1505.90 +/- 222.23, P < 0.05). The colonic MPO and MDA levels were significantly lower in control, BBS, NTS and BBS + NTS groups than in the colitis group (MPO levels, 24.36 +/- 8.10, 40.51 +/- 8.67 and 25.83 +/- 6.43 vs 161.47 +/- 38.24; MDA levels, 4.70 +/- 1.41, 6.55 +/- 1.12 and 4.51 +/- 0.54 vs 15.60 +/- 1.88, P < 0.05). Carbonyl content and caspase-3 levels were higher in the colitis and NTS groups than in control, BBS and BBS + NTS groups (carbonyl levels, 553.99 +/- 59.58 and 336.26 +/- 35.72 vs 209.76 +/- 30.92, 219.76 +/- 25.77 and 220.34 +/- 36.95; caspase-3 levels, 451.70 +/- 68.27 and 216.20 +/- 28.17 vs 28.60 +/- 6.46, 170.50 +/- 32.37 and 166.50 +/- 30.95, P < 0.05).

Conclusion: The results of this study suggest BBS and NTS, through their anti-inflammatory actions, support the maintenance of colonic integrity and merit consideration as potential agents for ameliorating colonic inflammation.

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Figures

Figure 1
Figure 1
Serum tumor necrosis factor-alpha (TNF-alpha) concentrations in rats. Vertical columns and error bars represent mean ± SD. bP < 0.001 vs BBS, NTS and BBS and NTS; aP < 0.05 vs control, colitis and NTS; cP < 0.05 vs control, colitis, BBS and BBS and NTS. BBS: Bombesin; NTS: Neurotensin.
Figure 2
Figure 2
Serum interleukin-6 (IL-6) concentrations in rats. Vertical columns and error bars represent mean ± SD. bP < 0.001 vs BBS, NTS and BBS&NTS; dP < 0.001 vs control, colitis and NTS; fP < 0.001 vs control, colitis and BBS and BBS and NTS. BBS: Bombesin; NTS: Neurotensin.
Figure 3
Figure 3
Colon myeloperoxidase (MPO) concentrations in rats. Vertical columns and error bars represent mean ± SD. bP < 0.001 vs colitis and NTS; dP < 0.001 vs control, BBS, NTS and BBS and NTS; fP < 0.001 vs colitis and NTS; cP < 0.05 vs control, colitis, BBS and BBS and NTS; eP < 0.05 vs control, colitis, BBS and NTS. BBS: Bombesin; NTS: Neurotensin.
Figure 4
Figure 4
Colon malondialdehyde (MDA) concentrations in rats. Vertical columns and error bars represent mean ± SD. bP < 0.001 vs colitis, NTS and BBS&NTS and aP < 0.05 vs BBS; dP < 0.001 vs control, BBS, NTS and BBS&NTS; fP < 0.001 vs colitis and eP < 0.05 vs control and NTS; hP < 0.001 vs control and colitis, jP < 0.05 vs BBS and BBS and NTS; kP < 0.001 vs control, colitis and NTS. BBS: Bombesin; NTS: Neurotensin.
Figure 5
Figure 5
Colonic carbonyl content in rats. Vertical columns and error bars represent mean ± SD. bP < 0.001 vs colitis and NTS; dP < 0.001 vs control, BBS, NTS and BBS&NTS; fP < 0.001 vs control, colitis, BBS and BBS&NTS; BBS: Bombesin; NTS: Neurotensin.
Figure 6
Figure 6
Colonic caspase-3 activity of all groups. Vertical columns and error bars represent mean ± SD. bP < 0.001 vs BBS, NTS and BBS&NTS; dP < 0.001 vs control, colitis and cP < 0.05 vs NTS; fP < 0.001 vs control and colitis, eP < 0.05 vs BBS and BBS and NTS; BBS: Bombesin; NTS: Neurotensin.
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