doi: 10.1016/j.jprot.2007.12.008.
Epub 2008 Jan 7.
Cyanine dyes for the detection of double stranded DNA
Affiliations
- PMID: 18304645
- DOI: 10.1016/j.jprot.2007.12.008
Item in Clipboard
Cyanine dyes for the detection of double stranded DNA
J Biochem Biophys Methods.
.
Abstract
Twenty three novel cyanine dyes have been applied as fluorescent stains for the detection of nucleic acids in agarose gel electrophoresis. Significant fluorescence enhancement of these dyes in the presence of double stranded DNA was observed. Five dyes offered superior sensitivity in the detection and quantification of DNA, over Ethidium Bromide, the most commonly used nucleic acid stain.
Similar articles
-
Studies of monomeric and homodimeric oxazolo[4,5-b]pyridinium cyanine dyes as fluorescent probes for nucleic acids visualization.J Biochem Biophys Methods. 2006 Oct 31;68(3):155-65. doi: 10.1016/j.jbbm.2006.04.006. Epub 2006 May 25. J Biochem Biophys Methods. 2006. PMID: 16828165
-
New amino and acetamido monomethine cyanine dyes for the detection of DNA in agarose gels.Bioorg Med Chem. 2007 Aug 15;15(16):5537-42. doi: 10.1016/j.bmc.2007.05.043. Epub 2007 May 23. Bioorg Med Chem. 2007. PMID: 17553684
-
Interaction of cyanine dyes with nucleic acids: XXXI. Using of polymethine cyanine dyes for the visualization of DNA in agarose gels.J Biochem Biophys Methods. 2003 Jul 31;57(1):35-43. doi: 10.1016/s0165-022x(03)00036-8. J Biochem Biophys Methods. 2003. PMID: 12834961
-
Symmetric cyanine dyes for detecting nucleic acids.Biotech Histochem. 2008 Jun;83(3-4):131-45. doi: 10.1080/10520290802383684. Biotech Histochem. 2008. PMID: 18802811 Review.
-
Cyanine dyes in biophysical research: the photophysics of polymethine fluorescent dyes in biomolecular environments.Q Rev Biophys. 2011 Feb;44(1):123-51. doi: 10.1017/S0033583510000247. Epub 2010 Nov 26. Q Rev Biophys. 2011. PMID: 21108866 Review.
Cited by
-
Spectroscopic study of the interaction of styrylcyanine dyes Sbo, Sil and their derivatives with bovine serum albumin.J Fluoresc. 2011 Jul;21(4):1713-9. doi: 10.1007/s10895-011-0866-7. Epub 2011 Mar 1. J Fluoresc. 2011. PMID: 21360296
-
Photonics of Trimethine Cyanine Dyes as Probes for Biomolecules.Molecules. 2022 Sep 27;27(19):6367. doi: 10.3390/molecules27196367. Molecules. 2022. PMID: 36234904 Free PMC article. Review.
-
Fluorescent probes for nucleic Acid visualization in fixed and live cells.Molecules. 2013 Dec 11;18(12):15357-97. doi: 10.3390/molecules181215357. Molecules. 2013. PMID: 24335616 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
