Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Jul 25;19(14):3857-60.
doi: 10.1093/nar/19.14.3857.

Genetic depletion indicates a late role for U5 snRNP during in vitro spliceosome assembly

B Séraphin  1 , N Abovich, M Rosbash
Affiliations
Free PMC article

Genetic depletion indicates a late role for U5 snRNP during in vitro spliceosome assembly

B Séraphin et al. Nucleic Acids Res. .
Free PMC article

Abstract

The pre-mRNA splicing pathway is highly conserved from yeast (S. cerevisiae) to mammals. Of the four snRNPs involved in splicing three (U1, U2 and U4/U6) have been shown to be essential for in vitro splicing. To examine the remaining snRNP, we utilized our previously described genetic procedures (Seraphin and Rosbash, 1989) to prepare yeast extracts depleted of U5 snRNP. The results show that U5 snRNP is necessary for both steps of pre- mRNA splicing and for proper spliceosome assembly, i.e., addition of the U4/U5/U6 triple snRNP. The prior steps of U1 and U2 snRNP addition occur normally in the absence of U5 snRNP.

PubMed Disclaimer

References

    1. EMBO J. 1991 May;10(5):1209-16 - PubMed
    1. Cell. 1984 Aug;38(1):299-307 - PubMed
    1. Genes Dev. 1990 Oct;4(10):1766-74 - PubMed
    1. Mol Cell Biol. 1990 Dec;10(12):6417-25 - PubMed
    1. Nucleic Acids Res. 1990 Dec 25;18(24):7373-9 - PubMed

Publication types