Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2008 Mar 19;582(6):997-1002.
doi: 10.1016/j.febslet.2008.02.047. Epub 2008 Feb 26.

Enhanced binding of TBK1 by an optineurin mutant that causes a familial form of primary open angle glaucoma

Simon Morton  1 , Luke Hesson, Mark Peggie, Philip Cohen
Affiliations
Free article

Enhanced binding of TBK1 by an optineurin mutant that causes a familial form of primary open angle glaucoma

Simon Morton et al. FEBS Lett. .
Free article

Abstract

TANK-binding kinase 1 (TBK1) was identified as a binding partner for Optineurin (OPTN) in two-hybrid screens, an interaction confirmed by overexpression/immunoprecipitation experiments in HEK293 cells and by coimmunoprecipitation of endogenous OPTN and TBK1 from cell extracts. A TBK1 binding site was located between residues 1-127 of OPTN, residues 78-121 displaying striking homology to the TBK1-binding domain of TANK. The OPTN-binding domain was localised to residues 601-729 of TBK1, while TBK1[1-688] which cannot bind to TANK, did not interact with OPTN. The OPTN[E50K] mutant associated with Primary Open Angle Glaucoma (POAG) displayed strikingly enhanced binding to TBK1, suggesting that this interaction may contribute to familial POAG caused by this mutation.

PubMed Disclaimer

Publication types

MeSH terms