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. 1976 Jul;2(6):445-54.
doi: 10.1007/BF00356933.

Interaction of aminoacyl-tRNA synthetases and tRNA: positive and negative cooperativity of their active centres

E G Malygin, V V Zinoviev, F Fasiolo, L L Kisselev, L L Kochkina, V Z Achverdyan

Interaction of aminoacyl-tRNA synthetases and tRNA: positive and negative cooperativity of their active centres

E G Malygin et al. Mol Biol Rep. 1976 Jul.

Abstract

The influence of tRNA on the kinetics of PP-ATP exchange and aminoacyl-tRNA formation catalysed by leucyl-, phenylalanyl-, and tryptophanyl-tRNA synthetases has been investigated. These enzymes were chosen because they belong to three main classes of quaternary structure alpha1, alpha2beta2 and alpha2, respectively. The present paper shows that the investigated synthetases manifest kinetic cooperativity of the active centres which is negative in the case of AAA formation and positive in the case of leucyl- and tryptophanyl-tRNA synthesis. The obtained data were interpreted with the aid of the trigger model of the enzyme.

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References

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