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. 2008 May 19;343(7):1237-50.
doi: 10.1016/j.carres.2008.01.042. Epub 2008 Feb 7.

Structural analysis of the alpha-D-glucan (EPS180) produced by the Lactobacillus reuteri strain 180 glucansucrase GTF180 enzyme

Sander S van Leeuwen  1 , Slavko Kralj, Ineke H van Geel-Schutten, Gerrit J Gerwig, Lubbert Dijkhuizen, Johannis P Kamerling
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Structural analysis of the alpha-D-glucan (EPS180) produced by the Lactobacillus reuteri strain 180 glucansucrase GTF180 enzyme

Sander S van Leeuwen et al. Carbohydr Res. .

Abstract

The neutral exopolysaccharide EPS180 produced from sucrose by the glucansucrase GTF180 enzyme from Lactobacillus reuteri 180 was found to be a (1-->3,1-->6)-alpha-D-glucan, with no repeating units present. Based on linkage analysis, periodate oxidation, and 1D/2D 1H and 13C NMR spectroscopy of the intact EPS180, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis of EPS180, a composite model, that includes all identified structural features, was formulated as follows: [Formula: see text].

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