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Review
. 2008 Mar;146(3):881-7.
doi: 10.1104/pp.107.111096.

Biotechnological prospects for engineering insect-resistant plants

John A Gatehouse  1
Affiliations
Review

Biotechnological prospects for engineering insect-resistant plants

John A Gatehouse. Plant Physiol. 2008 Mar.
No abstract available

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Figures

Figure 1.
Figure 1.
Engineering specificity in a three-domain Cry toxin; mutagenesis of the toxin-receptor interaction loop in domain II. Three-dimensional structure of Cry3A (1dlc; RCSB) is shown in ribbon format. Domain I (helices) is at top right, and domain II (sheet structure) is at bottom left. Domain III (carbohydrate-binding domain; sheet structure) is behind the other domains, central in this view. Residues mutated (Wu et al., 2000) to increase toxicity toward yellow mealworm (Tenebrio molitor), Colorado potato beetle, and cottonwood leaf beetle (Chrysomela scripta) are shown in ball-and stick representation. [See online article for color version of this figure.]
Figure 2.
Figure 2.
Engineering specificity in a three-domain Cry toxin; mutagenesis to improve channel-forming ability. Three-dimensional structure of Cry3Bb (1ji6; RCSB) is shown in ribbon format in the same view as Figure 1. Residues mutated (English et al., 2003) to increase toxicity toward corn rootworm are shown in ball-and-stick representation. Mutations are made in helices of domain I and in the region linking domains I and II. The mutation sites shown are taken from the most active toxin produced; a range of other sites for mutation were explored. [See online article for color version of this figure.]
See this image and copyright information in PMC

References

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